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KMID : 0545120100200111592
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 11 p.1592 ~ p.1596
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PCR-DGGE as a Supplemental Method Verifying Dominance of Culturable Microorganisms from Activated Sludge
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Sheng Zhou
Chaohai Wei
Lin Ke
Haizhen Wu
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Abstract
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To verify the dominance of microorganisms in wastewater biological treatment, PCR-DGGE (denaturing gradient gel electrophoresis) was performed as a supplementary support method for screening of the dominant microorganisms from activated sludge. Results suggest that the dominant microorganisms in activated sludge are primarily responsible for strengthening its effectiveness as a biological treatment system, followed by the non-main dominant microorganisms, whereas the non-dominant microorganisms showed no effects. The degree of microbial abundance present on the profile of PCR-DGGE was in line with the treatment efficiency of augmented activated sludge with isolated cultures, suggesting that PCR-DGGE can be used as an effective supplementary method for verifying culturable dominant microorganisms in activated sludge of coking wastewater.
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KEYWORD
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biological treatment, DNA, phenol-degrading microorganisms, PCR-DGGE, screening mean
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